Who are we and what do we want?
We are a bunch of scientists from Sheffield who are interested in understanding how neuronal circuits work. One of the ways to answer this fundamental question is to build neural circuits from scratch. We will therefore i) turn stem (NTERA-2) into neurons, make these neurons ii) grow and iii) form synapses with other neurons in a controlled way.
Has this been done before?
Yes, to some extent. There are many wonderful papers published on this topic and some of them will be discussed in this blog.
How do we differ?
Our approach is to marry classical biological techniques with machine learning techniques. By doing so, we will optimise the conditions for NTERA2 cells differentiation, regulation of axon growth and triggering long-term potentiation and depression. All this will be discussed in this blog.
What is this blog about?
Every individual experiment, it’s analysis and main results will be immediately published here. We will also discuss scientific ideas, research papers and share acquisition and analysis software (mostly written on Python).